Preprints of submitted manuscripts

Greene ER, Goodall EA, de la Peña AH, Matyskiela ME, Lander GC, Martin A. Specific lid-base contacts in the 26S proteasome control the conformational switching required for substrate engagement and degradation. bioRxiv 2019 July 2
Shin M, Asmita A, Puchades C, Adjei E, Wiseman RL, Karzai W, Lander GC. Distinct Structural Features of the Lon Protease Drive Conserved Hand-over-Hand Substrate Translocation. bioRxiv 2019 April 24
Puchades C, Ding B, Song A, Wiseman RL, Lander GC, Glynn SE. Unique structural features of the mitochondrial AAA+ protease AFG3L2 reveal the molecular basis for activity in health and disease. bioRxiv 2019 February 15

Peer-reviewed publications


Rollins MF, Chowdhury S, Carter J, Golden SM, Miettinen HM, Santiago-Frangos A, Faith D, Lawrence CM, Lander GC, Wiedenheft B. Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry. Mol Cell 2019 Apr 4; 74(1): 132-142.e5.

Associated preprint (posted 2018 October 26)
Herzik MA Jr, Wu M, Lander GC. High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM. Nat Commun 2019 Mar 4; 10(1): 1032.

Associated preprint (posted 2018 December 7)
Herzik MA Jr, Fraser JS, Lander GC. A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality. Structure 2019 Feb 5; 27(2): 344-358.e3.

Associated preprint (posted 2017 April 19)


Otomo T, Chowdhury S, Lander GC. The rod-shaped ATG2A-WIPI4 complex tethers membranes in vitro. Contact (Thousand Oaks) 2018 Jan-Dec; 1.
Zubcevic L, Herzik MA Jr, Wu M, Borschel WF, Hirschi M, Song AS, Lander GC, Lee SY. Conformational ensemble of the human TRPV3 ion channel. Nat Commun 2018 Nov 14; 9(1): 4773.

Associated preprint (posted 2018 August 20)
de la Pena AH, Goodall EA, Gates SN, Lander GC, Martin A. Substrate-engaged 26S proteasome structures reveal mechanisms for ATP-hydrolysis-driven translocation. Science 2018 Nov 30; 362(6418).

Associated preprint (posted 2018 August 16)
Chowdhury S, Otomo C, Leitner A, Ohashi K, Aebersold R, Lander GC, Otomo T. Insights into autophagosome biogenesis from structural and biochemical analyses of the ATG2A-WIPI4 complex. Proc Natl Acad Sci U S A 2018 Oct 16; 115(42): E9792-E9801.

Associated preprint (posted 2017 August 24)
Yoo J, Wu M, Yin Y, Herzik MA Jr, Lander GC, Lee SY. Cryo-EM structure of a mitochondrial calcium uniporter. Science 2018 Aug 3; 361(6401): 506-511.
Bruggemann J, Lander GC, Su AI. Exploring applications of crowdsourcing to cryo-EM. J Struct Biol 2018 Jul; 203(1): 37-45.

Associated preprint (posted 2017 November 15)
Grotjahn DA, Chowdhury S, Xu Y, McKenney RJ, Schroer TA, Lander GC. Cryo-electron tomography reveals that dynactin recruits a team of dyneins for processive motility. Nat Struct Mol Biol 2018 Mar; 25(3): 203-207.

Associated preprint (posted 2017 August 31)
Gardner BM, Castanzo DT, Chowdhury S, Stjepanovic G, Stefely MS, Hurley JH, Lander GC, Martin A. The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading. Nat Commun 2018 Jan 10; 9(1): 135.
Yin Y, Wu M, Zubcevic L, Borschel WF, Lander GC, Lee SY. Structure of the cold- and menthol-sensing ion channel TRPM8. Science 2018 Jan 12; 359(6372): 237-241.


Puchades C, Rampello AJ, Shin M, Giuliano CJ, Wiseman RL, Glynn SE, Lander GC. Structure of the mitochondrial inner membrane AAA+ protease YME1 gives insight into substrate processing. Science 2017 Nov 3; 358(6363).

Associated preprint (posted 2017 September 15)
Hirschi M, Herzik MA Jr, Wie J, Suo Y, Borschel WF, Ren D, Lander GC, Lee SY. Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3. Nature 2017 Oct 19; 550(7676): 411-414.
Herzik MA Jr, Wu M, Lander GC. Achieving better-than-3-A resolution by single-particle cryo-EM at 200 keV. Nat Methods 2017 Nov; 14(11): 1075-1078.

Associated preprint (posted 2017 May 25)
Chen KC, Qu S, Chowdhury S, Noxon IC, Schonhoft JD, Plate L, Powers ET, Kelly JW, Lander GC, Wiseman RL. The endoplasmic reticulum HSP40 co-chaperone ERdj3/DNAJB11 assembles and functions as a tetramer. EMBO J 2017 Aug 1; 36(15): 2296-2309.
Rollins MF, Chowdhury S, Carter J, Golden SM, Wilkinson RA, Bondy-Denomy J, Lander GC, Wiedenheft B. Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity. Proc Natl Acad Sci U S A 2017 Jun 27; 114(26): E5113-E5121.
Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B. Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex. Cell 2017 Mar 23; 169(1): 47-57.e11.


Smirnova IA, Sjostrand D, Li F, Bjorck M, Schafer J, Ostbye H, Hogbom M, von Ballmoos C, Lander GC, Adelroth P, Brzezinski P. Isolation of yeast complex IV in native lipid nanodiscs. Biochim Biophys Acta 2016 Dec; 1858(12): 2984-2992.
Matyskiela ME, Lu G, Ito T, Pagarigan B, Lu CC, Miller K, Fang W, Wang NY, Nguyen D, Houston J, Carmel G, Tran T, Riley M, Nosaka L, Lander GC, Gaidarova S, Xu S, Ruchelman AL, Handa H, Carmichael J, Daniel TO, Cathers BE, Lopez-Girona A, Chamberlain PP. A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase. Nature 2016 Jul 14; 535(7611): 252-7.
Zubcevic L, Herzik MA Jr, Chung BC, Liu Z, Lander GC, Lee SY. Cryo-electron microscopy structure of the TRPV2 ion channel. Nat Struct Mol Biol 2016 Feb; 23(2): 180-186.
Dambacher CM, Worden EJ, Herzik MA, Martin A, Lander GC. Atomic structure of the 26S proteasome lid reveals the mechanism of deubiquitinase inhibition. Elife 2016 Jan 8; 5: e13027.


Yang TC, Ortiz D, Nosaka L, Lander GC, Catalano CE. Thermodynamic Interrogation of the Assembly of a Viral Genome Packaging Motor Complex. Biophys J 2015 Oct 20; 109(8): 1663-75.
Ciferri C, Lander GC, Nogales E. Protein domain mapping by internal labeling and single particle electron microscopy. J Struct Biol 2015 Nov; 192(2): 159-62.
Dambacher CM, Lander GC. Site-specific labeling of proteins for electron microscopy. J Struct Biol 2015 Nov; 192(2): 151-8.
Bashore C, Dambacher CM, Goodall EA, Matyskiela ME, Lander GC, Martin A. Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome. Nat Struct Mol Biol 2015 Sep; 22(9): 712-9.
McNulty R, Lokareddy RK, Roy A, Yang Y, Lander GC, Heck AJR, Johnson JE, Cingolani G. Architecture of the Complex Formed by Large and Small Terminase Subunits from Bacteriophage P22. J Mol Biol 2015 Oct 9; 427(20): 3285-3299.
Garnham CP, Vemu A, Wilson-Kubalek EM, Yu I, Szyk A, Lander GC, Milligan RA, Roll-Mecak A. Multivalent Microtubule Recognition by Tubulin Tyrosine Ligase-like Family Glutamylases. Cell 2015 May 21; 161(5): 1112-1123.
Chowdhury S, Ketcham SA, Schroer TA, Lander GC. Structural organization of the dynein-dynactin complex bound to microtubules. Nat Struct Mol Biol 2015 Apr; 22(4): 345-7.
Gardner BM, Chowdhury S, Lander GC, Martin A. The Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits. J Mol Biol 2015 Mar 27; 427(6 Pt B): 1375-1388.


Liu T, Sae-Ueng U, Li D, Lander GC, Zuo X, Jonsson B, Rau D, Shefer I, Evilevitch A. Solid-to-fluid-like DNA transition in viruses facilitates infection. Proc Natl Acad Sci U S A 2014 Oct 14; 111(41): 14675-80.
Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E. High-resolution microtubule structures reveal the structural transitions in alphabeta-tubulin upon GTP hydrolysis. Cell 2014 May 22; 157(5): 1117-29.
Zhao Q, Potter CS, Carragher B, Lander G, Sworen J, Towne V, Abraham D, Duncan P, Washabaugh MW, Sitrin RD. Characterization of virus-like particles in GARDASIL(R) by cryo transmission electron microscopy. Hum Vaccin Immunother 2014; 10(3): 734-9.


Matyskiela ME, Lander GC, Martin A. Conformational switching of the 26S proteasome enables substrate degradation. Nat Struct Mol Biol 2013 Jul; 20(7): 781-8.
Lander GC, Johnson JE, Rau DC, Potter CS, Carragher B, Evilevitch A. DNA bending-induced phase transition of encapsidated genome in phage lambda. Nucleic Acids Res 2013 Apr; 41(8): 4518-24.
Lander GC, Martin A, Nogales E. The proteasome under the microscope: the regulatory particle in focus. Curr Opin Struct Biol 2013 Apr; 23(2): 243-51.


Ciferri C, Lander GC, Maiolica A, Herzog F, Aebersold R, Nogales E. Molecular architecture of human polycomb repressive complex 2. Elife 2012 Oct 30; 1: e00005.
Lander GC, Saibil HR, Nogales E. Go hybrid: EM, crystallography, and beyond. Curr Opin Struct Biol 2012 Oct; 22(5): 627-35.
Roy A, Bhardwaj A, Datta P, Lander GC, Cingolani G. Small terminase couples viral DNA binding to genome-packaging ATPase activity. Structure 2012 Aug 8; 20(8): 1403-13.
Lander GC, Baudoux AC, Azam F, Potter CS, Carragher B, Johnson JE. Capsomer dynamics and stabilization in the T = 12 marine bacteriophage SIO-2 and its procapsid studied by CryoEM. Structure 2012 Mar 7; 20(3): 498-503.
Lander GC, Estrin E, Matyskiela ME, Bashore C, Nogales E, Martin A. Complete subunit architecture of the proteasome regulatory particle. Nature 2012 Jan 11; 482(7384): 186-91.
Baudoux AC, Hendrix RW, Lander GC, Bailly X, Podell S, Paillard C, Johnson JE, Potter CS, Carragher B, Azam F. Genomic and functional analysis of Vibrio phage SIO-2 reveals novel insights into ecology and evolution of marine siphoviruses. Environ Microbiol 2012 Aug; 14(8): 2071-86.


Wiedenheft B, Lander GC, Zhou K, Jore MM, Brouns SJJ, van der Oost J, Doudna JA, Nogales E. Structures of the RNA-guided surveillance complex from a bacterial immune system. Nature 2011 Sep 21; 477(7365): 486-489.
Tang J, Lander GC, Olia AS, Li R, Casjens S, Prevelige P Jr, Cingolani G, Baker TS, Johnson JE. Peering down the barrel of a bacteriophage portal: the genome packaging and release valve in p22. Structure 2011 Apr 13; 19(4): 496-502.
Hornung P, Maier M, Alushin GM, Lander GC, Nogales E, Westermann S. Molecular architecture and connectivity of the budding yeast Mtw1 kinetochore complex. J Mol Biol 2011 Jan 14; 405(2): 548-59.


Huo Y, Hu Z, Zhang K, Wang L, Zhai Y, Zhou Q, Lander G, Zhu J, He Y, Pang X, Xu W, Bartlam M, Dong Z, Sun F. Crystal structure of group II chaperonin in the open state. Structure 2010 Oct 13; 18(10): 1270-9.
Matsui T, Lander GC, Khayat R, Johnson JE. Subunits fold at position-dependent rates during maturation of a eukaryotic RNA virus. Proc Natl Acad Sci U S A 2010 Aug 10; 107(32): 14111-5.
Khayat R, Lander GC, Johnson JE. An automated procedure for detecting protein folds from sub-nanometer resolution electron density. J Struct Biol 2010 Jun; 170(3): 513-21.
Voss NR, Lyumkis D, Cheng A, Lau PW, Mulder A, Lander GC, Brignole EJ, Fellmann D, Irving C, Jacovetty EL, Leung A, Pulokas J, Quispe JD, Winkler H, Yoshioka C, Carragher B, Potter CS. A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy. J Struct Biol 2010 Mar; 169(3): 389-98.


Lander GC, Khayat R, Li R, Prevelige PE, Potter CS, Carragher B, Johnson JE. The P22 tail machine at subnanometer resolution reveals the architecture of an infection conduit. Structure 2009 Jun 10; 17(6): 789-99.
Ambrose RL, Lander GC, Maaty WS, Bothner B, Johnson JE, Johnson KN. Drosophila A virus is an unusual RNA virus with a T=3 icosahedral core and permuted RNA-dependent RNA polymerase. J Gen Virol 2009 Sep; 90(Pt 9): 2191-200.
Lander GC, Stagg SM, Voss NR, Cheng A, Fellmann D, Pulokas J, Yoshioka C, Irving C, Mulder A, Lau PW, Lyumkis D, Potter CS, Carragher B. Appion: an integrated, database-driven pipeline to facilitate EM image processing. J Struct Biol 2009 Apr; 166(1): 95-102.


Lander GC, Evilevitch A, Jeembaeva M, Potter CS, Carragher B, Johnson JE. Bacteriophage lambda stabilization by auxiliary protein gpD: timing, location, and mechanism of attachment determined by cryo-EM. Structure 2008 Sep 10; 16(9): 1399-406.
Nemecek D, Lander GC, Johnson JE, Casjens SR, Thomas GJ Jr. Assembly architecture and DNA binding of the bacteriophage P22 terminase small subunit. J Mol Biol 2008 Nov 14; 383(3): 494-501.
Stagg SM, Lander GC, Quispe J, Voss NR, Cheng A, Bradlow H, Bradlow S, Carragher B, Potter CS. A test-bed for optimizing high-resolution single particle reconstructions. J Struct Biol 2008 Jul; 163(1): 29-39.
Prasuhn DE Jr, Kuzelka J, Strable E, Udit AK, Cho SH, Lander GC, Quispe JD, Diers JR, Bocian DF, Potter C, Carragher B, Finn MG. Polyvalent display of heme on hepatitis B virus capsid protein through coordination to hexahistidine tags. Chem Biol 2008 May; 15(5): 513-9.
Strable E, Prasuhn DE Jr, Udit AK, Brown S, Link AJ, Ngo JT, Lander G, Quispe J, Potter CS, Carragher B, Tirrell DA, Finn MG. Unnatural amino acid incorporation into virus-like particles. Bioconjug Chem 2008 Apr; 19(4): 866-75.


Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE. Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM. Structure 2006 Nov; 14(11): 1655-65.


Poliakov A, van Duijn E, Lander G, Fu CY, Johnson JE, Prevelige PE Jr, Heck AJ. Macromolecular mass spectrometry and electron microscopy as complementary tools for investigation of the heterogeneity of bacteriophage portal assemblies. J Struct Biol 2007 Feb; 157(2): 371-83.


Stagg SM, Lander GC, Pulokas J, Fellmann D, Cheng A, Quispe JD, Mallick SP, Avila RM, Carragher B, Potter CS. Automated cryoEM data acquisition and analysis of 284742 particles of GroEL. J Struct Biol 2006 Sep; 155(3): 470-81.
Lander GC, Tang L, Casjens SR, Gilcrease EB, Prevelige P, Poliakov A, Potter CS, Carragher B, Johnson JE. The structure of an infectious P22 virion shows the signal for headful DNA packaging. Science 2006 Jun 23; 312(5781): 1791-5.
Shepherd CM, Borelli IA, Lander G, Natarajan P, Siddavanahalli V, Bajaj C, Johnson JE, Brooks CL 3rd, Reddy VS. VIPERdb: a relational database for structural virology. Nucleic Acids Res 2006 Jan 1; 34(Database issue): D386-9.


Natarajan P, Lander GC, Shepherd CM, Reddy VS, Brooks CL 3rd, Johnson JE. Exploring icosahedral virus structures with VIPER. Nat Rev Microbiol 2005 Oct; 3(10): 809-17.


Berardini TZ, Mundodi S, Reiser L, Huala E, Garcia-Hernandez M, Zhang P, Mueller LA, Yoon J, Doyle A, Lander G, Moseyko N, Yoo D, Xu I, Zoeckler B, Montoya M, Miller N, Weems D, Rhee SY. Functional annotation of the Arabidopsis genome using controlled vocabularies. Plant Physiol 2004 Jun; 135(2): 745-55.


Rhee SY, Beavis W, Berardini TZ, Chen G, Dixon D, Doyle A, Garcia-Hernandez M, Huala E, Lander G, Montoya M, Miller N, Mueller LA, Mundodi S, Reiser L, Tacklind J, Weems DC, Wu Y, Xu I, Yoo D, Yoon J, Zhang P. The Arabidopsis Information Resource (TAIR): a model organism database providing a centralized, curated gateway to Arabidopsis biology, research materials and community. Nucleic Acids Res 2003 Jan 1; 31(1): 224-8.