Preprints of submitted manuscripts

Protein dynamics regulate distinct biochemical properties of cryptochromes in mammalian circadian rhythms
Fribourgh JL, Srivastava A, Sandate CR, Michael AK, Hsu PL, Rakers C, Nguyen LT, Torgrimson MR, Parico GCG, Tripathi S, Zheng N, Lander GC, Hirota T, Tama F, Partch CL
Online August 20, 2019
bioRxiv DOI: 10.1101/740464
Specific lid-base contacts in the 26S proteasome control the conformational switching required for substrate engagement and degradation
Greene ER, Goodall EA, de la Peña AH, Matyskiela ME, Lander GC, Martin A
Online July 2, 2019
bioRxiv DOI: 10.1101/687921
Distinct Structural Features of the Lon Protease Drive Conserved Hand-over-Hand Substrate Translocation
Shin M, Asmita A, Puchades C, Adjei E, Wiseman RL, Karzai AW, Lander GC
Online April 24, 2019
bioRxiv DOI: 10.1101/617159

Peer-reviewed publications

Journal names have been removed from the list below.
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Current outcomes when optimizing "standard" sample preparation for single-particle cryo-em.
Carragher B, Cheng Y, Frost A, Glaeser RM, Lander GC, Nogales E, Wang HW
Published Sep 25, 2019
  • PMID: 31553060
  • Visualizing structural transitions of ligand-dependent gating of the TRPM2 channel.
    Yin Y, Wu M, Hsu AL, Borschel WF, Borgnia MJ, Lander GC, Lee SY
    Published Aug 20, 2019
  • PMID: 31431622
  • PMCID: 6702222
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  • Associated preprint (posted January 9, 2019)
    Unique Structural Features of the Mitochondrial AAA+ Protease AFG3L2 Reveal the Molecular Basis for Activity in Health and Disease.
    Puchades C, Ding B, Song A, Wiseman RL, Lander GC, Glynn SE
    Published Sep 5, 2019
  • PMID: 31327635
  • PMCID: 6731152
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  • Associated preprint (posted February 15, 2019)
    An allosteric network in spastin couples multiple activities required for microtubule severing.
    Sandate CR, Szyk A, Zehr EA, Lander GC, Roll-Mecak A
    Published Aug 2019
  • PMID: 31285604
  • PMCID: 6761829
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  • Setting the dynein motor in motion: New insights from electron tomography.
    Grotjahn DA, Lander GC
    Published Sep 6, 2019
  • PMID: 31285262
  • PMCID: 6737236
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  • Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry.
    Rollins MF, Chowdhury S, Carter J, Golden SM, Miettinen HM, Santiago-Frangos A, Faith D, Lawrence CM, Lander GC, Wiedenheft B
    Published Apr 4, 2019
  • PMID: 30872121
  • PMCID: 6521718
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  • Associated preprint (posted October 26, 2018)
    High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM.
    Herzik MA Jr, Wu M, Lander GC
    Published Mar 4, 2019
  • PMID: 30833564
  • PMCID: 6399227
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  • Associated preprint (posted December 7, 2018)
    A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality.
    Herzik MA Jr, Fraser JS, Lander GC
    Published Feb 5, 2019
  • PMID: 30449687
  • PMCID: 6365196
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  • Associated preprint (posted April 19, 2017)


    The rod-shaped ATG2A-WIPI4 complex tethers membranes in vitro.
    Otomo T, Chowdhury S, Lander GC
    Published Jan-Dec 2018
  • PMID: 30766969
  • PMCID: 6372110
  • Conformational ensemble of the human TRPV3 ion channel.
    Zubcevic L, Herzik MA Jr, Wu M, Borschel WF, Hirschi M, Song AS, Lander GC, Lee SY
    Published Nov 14, 2018
  • PMID: 30429472
  • PMCID: 6235889
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  • Associated preprint (posted August 20, 2018)
    Substrate-engaged 26S proteasome structures reveal mechanisms for ATP-hydrolysis-driven translocation.
    de la Pena AH, Goodall EA, Gates SN, Lander GC, Martin A
    Published Nov 30, 2018
  • PMID: 30309908
  • PMCID: 6519459
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  • Associated preprint (posted August 16, 2018)
    Insights into autophagosome biogenesis from structural and biochemical analyses of the ATG2A-WIPI4 complex.
    Chowdhury S, Otomo C, Leitner A, Ohashi K, Aebersold R, Lander GC, Otomo T
    Published Oct 16, 2018
  • PMID: 30185561
  • PMCID: 6196511
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  • Associated preprint (posted August 24, 2017)
    Cryo-EM structure of a mitochondrial calcium uniporter.
    Yoo J, Wu M, Yin Y, Herzik MA Jr, Lander GC, Lee SY
    Published Aug 3, 2018
  • PMID: 29954988
  • PMCID: 6155975
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  • Exploring applications of crowdsourcing to cryo-EM.
    Bruggemann J, Lander GC, Su AI
    Published Jul 2018
  • PMID: 29486249
  • PMCID: 6086358
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  • Associated preprint (posted November 15, 2017)
    Cryo-electron tomography reveals that dynactin recruits a team of dyneins for processive motility.
    Grotjahn DA, Chowdhury S, Xu Y, McKenney RJ, Schroer TA, Lander GC
    Published Mar 2018
  • PMID: 29416113
  • PMCID: 5969528
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  • Associated preprint (posted August 31, 2017)
    The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading.
    Gardner BM, Castanzo DT, Chowdhury S, Stjepanovic G, Stefely MS, Hurley JH, Lander GC, Martin A
    Published Jan 10, 2018
  • PMID: 29321502
  • PMCID: 5762779
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  • Structure of the cold- and menthol-sensing ion channel TRPM8.
    Yin Y, Wu M, Zubcevic L, Borschel WF, Lander GC, Lee SY
    Published Jan 12, 2018
  • PMID: 29217583
  • PMCID: 5810135
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  • 2017

    Structure of the mitochondrial inner membrane AAA+ protease YME1 gives insight into substrate processing.
    Puchades C, Rampello AJ, Shin M, Giuliano CJ, Wiseman RL, Glynn SE, Lander GC
    Published Nov 3, 2017
  • PMID: 29097521
  • PMCID: 5829300
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  • Associated preprint (posted September 15, 2017)
    Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3.
    Hirschi M, Herzik MA Jr, Wie J, Suo Y, Borschel WF, Ren D, Lander GC, Lee SY
    Published Oct 19, 2017
  • PMID: 29019979
  • PMCID: 5762132
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  • Achieving better-than-3-A resolution by single-particle cryo-EM at 200 keV.
    Herzik MA Jr, Wu M, Lander GC
    Published Nov 2017
  • PMID: 28991891
  • PMCID: 5679434
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  • Associated preprint (posted May 25, 2017)
    The endoplasmic reticulum HSP40 co-chaperone ERdj3/DNAJB11 assembles and functions as a tetramer.
    Chen KC, Qu S, Chowdhury S, Noxon IC, Schonhoft JD, Plate L, Powers ET, Kelly JW, Lander GC, Wiseman RL
    Published Aug 1, 2017
  • PMID: 28655754
  • PMCID: 5538767
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  • Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity.
    Rollins MF, Chowdhury S, Carter J, Golden SM, Wilkinson RA, Bondy-Denomy J, Lander GC, Wiedenheft B
    Published Jun 27, 2017
  • PMID: 28438998
  • PMCID: 5495223
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  • Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex.
    Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B
    Published Mar 23, 2017
  • PMID: 28340349
  • PMCID: 5478280
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  • 2016

    What's the Key to Unlocking the Proteasome's Gate?
    de la Pena AH, Lander GC
    Published Dec 6, 2016
  • PMID: 27926830
  • Isolation of yeast complex IV in native lipid nanodiscs.
    Smirnova IA, Sjostrand D, Li F, Bjorck M, Schafer J, Ostbye H, Hogbom M, von Ballmoos C, Lander GC, Adelroth P, Brzezinski P
    Published Dec 2016
  • PMID: 27620332
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  • A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase.
    Matyskiela ME, Lu G, Ito T, Pagarigan B, Lu CC, Miller K, Fang W, Wang NY, Nguyen D, Houston J, Carmel G, Tran T, Riley M, Nosaka L, Lander GC, Gaidarova S, Xu S, Ruchelman AL, Handa H, Carmichael J, Daniel TO, Cathers BE, Lopez-Girona A, Chamberlain PP
    Published Jul 14, 2016
  • PMID: 27338790
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  • Cryo-electron microscopy structure of the TRPV2 ion channel.
    Zubcevic L, Herzik MA Jr, Chung BC, Liu Z, Lander GC, Lee SY
    Published Feb 2016
  • PMID: 26779611
  • PMCID: 4876856
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  • Atomic structure of the 26S proteasome lid reveals the mechanism of deubiquitinase inhibition.
    Dambacher CM, Worden EJ, Herzik MA, Martin A, Lander GC
    Published Jan 8, 2016
  • PMID: 26744777
  • PMCID: 4749569
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  • 2015

    Thermodynamic Interrogation of the Assembly of a Viral Genome Packaging Motor Complex.
    Yang TC, Ortiz D, Nosaka L, Lander GC, Catalano CE
    Published Oct 20, 2015
  • PMID: 26488657
  • PMCID: 4624345
  • Protein domain mapping by internal labeling and single particle electron microscopy.
    Ciferri C, Lander GC, Nogales E
    Published Nov 2015
  • PMID: 26431894
  • PMCID: 4921062
  • Site-specific labeling of proteins for electron microscopy.
    Dambacher CM, Lander GC
    Published Nov 2015
  • PMID: 26409249
  • PMCID: 4633364
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  • Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome.
    Bashore C, Dambacher CM, Goodall EA, Matyskiela ME, Lander GC, Martin A
    Published Sep 2015
  • PMID: 26301997
  • PMCID: 4560640
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  • Architecture of the Complex Formed by Large and Small Terminase Subunits from Bacteriophage P22.
    McNulty R, Lokareddy RK, Roy A, Yang Y, Lander GC, Heck AJR, Johnson JE, Cingolani G
    Published Oct 9, 2015
  • PMID: 26301600
  • PMCID: 4587339
  • Multivalent Microtubule Recognition by Tubulin Tyrosine Ligase-like Family Glutamylases.
    Garnham CP, Vemu A, Wilson-Kubalek EM, Yu I, Szyk A, Lander GC, Milligan RA, Roll-Mecak A
    Published May 21, 2015
  • PMID: 25959773
  • PMCID: 4465277
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  • Structural organization of the dynein-dynactin complex bound to microtubules.
    Chowdhury S, Ketcham SA, Schroer TA, Lander GC
    Published Apr 2015
  • PMID: 25751425
  • PMCID: 4385409
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  • The Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits.
    Gardner BM, Chowdhury S, Lander GC, Martin A
    Published Mar 27, 2015
  • PMID: 25659908
  • PMCID: 4355278
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  • 2014

    Solid-to-fluid-like DNA transition in viruses facilitates infection.
    Liu T, Sae-Ueng U, Li D, Lander GC, Zuo X, Jonsson B, Rau D, Shefer I, Evilevitch A
    Published Oct 14, 2014
  • PMID: 25271319
  • PMCID: 4205597
  • High-resolution microtubule structures reveal the structural transitions in alphabeta-tubulin upon GTP hydrolysis.
    Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E
    Published May 22, 2014
  • PMID: 24855948
  • PMCID: 4054694
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  • Characterization of virus-like particles in GARDASIL(R) by cryo transmission electron microscopy.
    Zhao Q, Potter CS, Carragher B, Lander G, Sworen J, Towne V, Abraham D, Duncan P, Washabaugh MW, Sitrin RD
    Published 2014
  • PMID: 24299977
  • PMCID: 4130261

  • 2013

    Conformational switching of the 26S proteasome enables substrate degradation.
    Matyskiela ME, Lander GC, Martin A
    Published Jul 2013
  • PMID: 23770819
  • PMCID: 3712289
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  • The proteasome under the microscope: the regulatory particle in focus.
    Lander GC, Martin A, Nogales E
    Published Apr 2013
  • PMID: 23498601
  • PMCID: 3676703
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  • DNA bending-induced phase transition of encapsidated genome in phage lambda.
    Lander GC, Johnson JE, Rau DC, Potter CS, Carragher B, Evilevitch A
    Published Apr 2013
  • PMID: 23449219
  • PMCID: 3632124
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  • 2012

    Molecular architecture of human polycomb repressive complex 2.
    Ciferri C, Lander GC, Maiolica A, Herzog F, Aebersold R, Nogales E
    Published Oct 30, 2012
  • PMID: 23110252
  • PMCID: 3482686
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  • Go hybrid: EM, crystallography, and beyond.
    Lander GC, Saibil HR, Nogales E
    Published Oct 2012
  • PMID: 22835744
  • PMCID: 3478466
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  • Small terminase couples viral DNA binding to genome-packaging ATPase activity.
    Roy A, Bhardwaj A, Datta P, Lander GC, Cingolani G
    Published Aug 8, 2012
  • PMID: 22771211
  • PMCID: 3563279
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  • Capsomer dynamics and stabilization in the T = 12 marine bacteriophage SIO-2 and its procapsid studied by CryoEM.
    Lander GC, Baudoux AC, Azam F, Potter CS, Carragher B, Johnson JE
    Published Mar 7, 2012
  • PMID: 22405008
  • PMCID: 3302160
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  • Complete subunit architecture of the proteasome regulatory particle.
    Lander GC, Estrin E, Matyskiela ME, Bashore C, Nogales E, Martin A
    Published Jan 11, 2012
  • PMID: 22237024
  • PMCID: 3285539
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  • Genomic and functional analysis of Vibrio phage SIO-2 reveals novel insights into ecology and evolution of marine siphoviruses.
    Baudoux AC, Hendrix RW, Lander GC, Bailly X, Podell S, Paillard C, Johnson JE, Potter CS, Carragher B, Azam F
    Published Aug 2012
  • PMID: 22225728
  • PMCID: 3338904
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  • 2011

    Structures of the RNA-guided surveillance complex from a bacterial immune system.
    Wiedenheft B, Lander GC, Zhou K, Jore MM, Brouns SJJ, van der Oost J, Doudna JA, Nogales E
    Published Sep 21, 2011
  • PMID: 21938068
  • PMCID: 4165517
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  • Peering down the barrel of a bacteriophage portal: the genome packaging and release valve in p22.
    Tang J, Lander GC, Olia AS, Li R, Casjens S, Prevelige P Jr, Cingolani G, Baker TS, Johnson JE
    Published Apr 13, 2011
  • PMID: 21439834
  • PMCID: 3075339
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  • Molecular architecture and connectivity of the budding yeast Mtw1 kinetochore complex.
    Hornung P, Maier M, Alushin GM, Lander GC, Nogales E, Westermann S
    Published Jan 14, 2011
  • PMID: 21075115
  • PMCID: 3017645
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  • 2010

    Crystal structure of group II chaperonin in the open state.
    Huo Y, Hu Z, Zhang K, Wang L, Zhai Y, Zhou Q, Lander G, Zhu J, He Y, Pang X, Xu W, Bartlam M, Dong Z, Sun F
    Published Oct 13, 2010
  • PMID: 20947016
  • PMCID: 3048791
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  • Subunits fold at position-dependent rates during maturation of a eukaryotic RNA virus.
    Matsui T, Lander GC, Khayat R, Johnson JE
    Published Aug 10, 2010
  • PMID: 20660783
  • PMCID: 2922577
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  • An automated procedure for detecting protein folds from sub-nanometer resolution electron density.
    Khayat R, Lander GC, Johnson JE
    Published Jun 2010
  • PMID: 20026407
  • PMCID: 2866771
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  • A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy.
    Voss NR, Lyumkis D, Cheng A, Lau PW, Mulder A, Lander GC, Brignole EJ, Fellmann D, Irving C, Jacovetty EL, Leung A, Pulokas J, Quispe JD, Winkler H, Yoshioka C, Carragher B, Potter CS
    Published Mar 2010
  • PMID: 20018246
  • PMCID: 2826578
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  • 2009

    The P22 tail machine at subnanometer resolution reveals the architecture of an infection conduit.
    Lander GC, Khayat R, Li R, Prevelige PE, Potter CS, Carragher B, Johnson JE
    Published Jun 10, 2009
  • PMID: 19523897
  • PMCID: 2714705
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  • Drosophila A virus is an unusual RNA virus with a T=3 icosahedral core and permuted RNA-dependent RNA polymerase.
    Ambrose RL, Lander GC, Maaty WS, Bothner B, Johnson JE, Johnson KN
    Published Sep 2009
  • PMID: 19474243
  • PMCID: 2742409
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  • Ab initio maximum likelihood reconstruction from cryo electron microscopy images of an infectious virion of the tailed bacteriophage P22 and maximum likelihood versions of Fourier Shell Correlation appropriate for measuring resolution of spherical or cylindrical objects.
    Prust CJ, Doerschuk PC, Lander GC, Johnson JE
    Published Sep 2009
  • PMID: 19457456
  • PMCID: 2803348
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  • Appion: an integrated, database-driven pipeline to facilitate EM image processing.
    Lander GC, Stagg SM, Voss NR, Cheng A, Fellmann D, Pulokas J, Yoshioka C, Irving C, Mulder A, Lau PW, Lyumkis D, Potter CS, Carragher B
    Published Apr 2009
  • PMID: 19263523
  • PMCID: 2775544
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  • Characterization of large conformational changes and autoproteolysis in the maturation of a T=4 virus capsid.
    Matsui T, Lander G, Johnson JE
    Published Jan 2009
  • PMID: 18987141
  • PMCID: 2612371

  • 2008

    Bacteriophage lambda stabilization by auxiliary protein gpD: timing, location, and mechanism of attachment determined by cryo-EM.
    Lander GC, Evilevitch A, Jeembaeva M, Potter CS, Carragher B, Johnson JE
    Published Sep 10, 2008
  • PMID: 18786402
  • PMCID: 2614288
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  • Assembly architecture and DNA binding of the bacteriophage P22 terminase small subunit.
    Nemecek D, Lander GC, Johnson JE, Casjens SR, Thomas GJ Jr
    Published Nov 14, 2008
  • PMID: 18775728
  • PMCID: 2577774
  • A test-bed for optimizing high-resolution single particle reconstructions.
    Stagg SM, Lander GC, Quispe J, Voss NR, Cheng A, Bradlow H, Bradlow S, Carragher B, Potter CS
    Published Jul 2008
  • PMID: 18534866
  • PMCID: 2505049
  • Polyvalent display of heme on hepatitis B virus capsid protein through coordination to hexahistidine tags.
    Prasuhn DE Jr, Kuzelka J, Strable E, Udit AK, Cho SH, Lander GC, Quispe JD, Diers JR, Bocian DF, Potter C, Carragher B, Finn MG
    Published May 2008
  • PMID: 18482703
  • PMCID: 2715361
  • Unnatural amino acid incorporation into virus-like particles.
    Strable E, Prasuhn DE Jr, Udit AK, Brown S, Link AJ, Ngo JT, Lander G, Quispe J, Potter CS, Carragher B, Tirrell DA, Finn MG
    Published Apr 2008
  • PMID: 18318461
  • PMCID: 2713011
  • Development of bacteriophage p22 as a platform for molecular display: genetic and chemical modifications of the procapsid exterior surface.
    Kang S, Lander GC, Johnson JE, Prevelige PE
    Published Mar 3, 2008
  • PMID: 18213564

  • 2006

    Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM.
    Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE
    Published Nov 2006
  • PMID: 17098191

  • 2007

    Macromolecular mass spectrometry and electron microscopy as complementary tools for investigation of the heterogeneity of bacteriophage portal assemblies.
    Poliakov A, van Duijn E, Lander G, Fu CY, Johnson JE, Prevelige PE Jr, Heck AJ
    Published Feb 2007
  • PMID: 17064935

  • 2006

    Automated cryoEM data acquisition and analysis of 284742 particles of GroEL.
    Stagg SM, Lander GC, Pulokas J, Fellmann D, Cheng A, Quispe JD, Mallick SP, Avila RM, Carragher B, Potter CS
    Published Sep 2006
  • PMID: 16762565
  • The structure of an infectious P22 virion shows the signal for headful DNA packaging.
    Lander GC, Tang L, Casjens SR, Gilcrease EB, Prevelige P, Poliakov A, Potter CS, Carragher B, Johnson JE
    Published Jun 23, 2006
  • PMID: 16709746
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  • VIPERdb: a relational database for structural virology.
    Shepherd CM, Borelli IA, Lander G, Natarajan P, Siddavanahalli V, Bajaj C, Johnson JE, Brooks CL 3rd, Reddy VS
    Published Jan 1, 2006
  • PMID: 16381893
  • PMCID: 1347395

  • 2005

    Exploring icosahedral virus structures with VIPER.
    Natarajan P, Lander GC, Shepherd CM, Reddy VS, Brooks CL 3rd, Johnson JE
    Published Oct 2005
  • PMID: 16205712
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  • 2004

    Functional annotation of the Arabidopsis genome using controlled vocabularies.
    Berardini TZ, Mundodi S, Reiser L, Huala E, Garcia-Hernandez M, Zhang P, Mueller LA, Yoon J, Doyle A, Lander G, Moseyko N, Yoo D, Xu I, Zoeckler B, Montoya M, Miller N, Weems D, Rhee SY
    Published Jun 2004
  • PMID: 15173566
  • PMCID: 514112

  • 2003

    The Arabidopsis Information Resource (TAIR): a model organism database providing a centralized, curated gateway to Arabidopsis biology, research materials and community.
    Rhee SY, Beavis W, Berardini TZ, Chen G, Dixon D, Doyle A, Garcia-Hernandez M, Huala E, Lander G, Montoya M, Miller N, Mueller LA, Mundodi S, Reiser L, Tacklind J, Weems DC, Wu Y, Xu I, Yoo D, Yoon J, Zhang P
    Published Jan 1, 2003
  • PMID: 12519987
  • PMCID: 165523